Tissue Culture Facility
Bernard Weissman, PhD, Faculty Director
Steve Oglesbee, Facility Director
The Tissue Culture Facility (TCF) is the UNC Lineberger's most comprehensive and widely used core facility. The TCF is a full-service cell culture shared resource and provides Cancer Center members and UNC colleagues with a wide variety of services and products at substantial discounts. Services and products include:
Media, Reagents, and Supplies:
The TCF stocks media, media supplements, serum, salt solutions, antibiotics, cell culture flasks and dishes and many other in vitro cell culture-related items.
Cell Culture Services:
The TCF offers a wide range of cell culture services from frozen vials and starter cultures to large-scale suspension systems of over 75 liters, specialized multiple-layer units for anchorage cells, and hollow-fiber bioreactors for monoclonal antibody or other cell metabolite production. Anchorage and suspension-type cells can be grown in most any size or volume to meet the researcher's needs.
Special Cell Culture Services:
The TCF's monoclonal antibody (Mab) production services are recommended by the UNC Institutional Animal Care and Use Committee (IACUC) as an alternative to Mab production in animal ascites. More detailed information. The TCF also can produce immortalized cell lines from human B lymphocytes using a protocol utilizing Epstein Barr virus (EBV). The TCF's current success rate in creating immortalized cell lines is 100%. More detailed information.
Consultation, Training and Technical Assistance:
The TCF offers individual consultation, training sessions, and training materials in cell and tissue culture techniques, including media and systems for in vitro propagation, testing, and cell cryopreservation. The staff is available for help in developmental work as well as cell culture problem solving.
Centralized Cell Repository:
Over 400 cell lines are available, as well as various computerized catalogues (American Type Culture Collection, NIGMS Human Genetic Mutant Cell Repository). Cell lines include normal and transformed mammalian, avian, insect, amphibian/reptile, and hybridoma cells. Complete cell cryopreservation services are available.
Screening and Testing:
The TCF tests cell cultures, serum, media, and media- related products for their quality, efficacy, and usefulness in research. Testing services include endotoxin, mycoplasma, and sterility tests. The TCF can also evaluate new products that may be useful and/or cost effective for facility users. In addition to these services, the TCF strives to work with Center members to meet their individualized research needs. Please consult with the staff about your particular requirements.
Molecular Biology Support Program
The Tissue Culture Facility operates the only centralized molecular biology materials program at UNC. Through the program, researchers can obtain almost any enzyme, reagent, or kit for use in molecular biology related work. The TCF has over 300 products from eight suppliers at its facility in the Lineberger building and at a satellite program in Fordham Hall. All of the products are discounted 15-45 percent.
In addition, any products not in stock can be ordered from these suppliers at the same discount rates, and products are shipped via overnight carrier with free shipping. The companies currently under contract with the Tissue Culture Facility are: Amersham/Pharmacia, Invitrogen (GIBCO/BRL), Promega, New England Biolabs, Roche Biochemicals, Sigma, and Stratagene.
Location: Room 318, UNC Lineberger Comprehensive Cancer Center
Monday - Friday, 8:00 a.m. - 12:00 p.m., 1:00 p.m.- 5:30 p.m.
The facility has a total of nine additional staff members including technicians and support personnel.